| 出品公司: | Promega |
|---|---|
| 载体名称: | pNL2.2[NlucP/Hygro] |
| 质粒类型: | 荧光素酶载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 4832 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
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pNL2.2[NlucP/Hygro]载体质粒基本信息
pNL2.2[NlucP/Hygro]质粒图谱载体图谱和pNL2.2[NlucP/Hygro]载体序列质粒序列多克隆位点信息
![pNL2.2[NlucPHygro]载体图谱](/uploads/载体图谱/pNL2.2[NlucPHygro]载体图谱.png)
pNL2.2[NlucP/Hygro]质粒载体简介
pNL2.2[NlucP/Hygro]质粒序列载体序列
LOCUS pNL2.2[NlucP Hygro] 4832 bp ds-DNA circular SYN 26-JUL-2013
DEFINITION Promoterless vector encoding destabilized NanoLuc(R) luciferase for
measuring the activity of promoter and enhancer sequences.
ACCESSION JQ513373
VERSION .
KEYWORDS pNL2.2[NlucP Hygro]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4832)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Saturday, June 6, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4832
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
CDS 100..612
/codon_start=1
/product="NanoLuc(R) luciferase"
/note="Nluc"
/note="human codon-optimized"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
CDS 616..735
/codon_start=1
/product="PEST degradation sequence from mouse ornithine
decarboxylase"
/note="hPEST"
/note="human codon-optimized"
/translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
polyA_signal 787..908
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 1102..1459
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1310..1445
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 1490..2527
/codon_start=1
/product="hygromycin B phosphotransferase"
/note="HygR"
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
EVGRV"
polyA_signal 2551..2599
/note="synthetic polyadenylation signal"
rep_origin complement(2926..3514)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3714..4574)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 4679..4727
/note="synthetic polyadenylation signal"
misc_feature 4741..4832
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggtcttcac actcgaagat
121 ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct tgaacaggga
181 ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc
241 ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta tgaaggtctg
301 agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat
361 catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt tacgccgaac
421 atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc
481 actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct gatcaacccc
541 gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg gctgtgcgaa
601 cgcattctgg cgaattctca cggctttccg cctgaggttg aagagcaagc cgccggtaca
661 ttgcctatgt cctgcgcaca agaaagcggt atggaccggc acccagccgc ttgtgcttca
721 gctcgcatca acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag
781 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
841 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
901 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
961 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtttg
1021 cgtattgggc gctcttccgc tgatctgcgc agcaccatgg cctgaaataa cctctgaaag
1081 aggaacttgg ttagctacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
1141 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
1201 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
1261 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
1321 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
1381 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
1441 aggcctaggc ttttgcaaaa agctcgattc ttctgacact agcgccacca tgaagaagcc
1501 cgaactcacc gctaccagcg ttgaaaaatt tctcatcgag aagttcgaca gtgtgagcga
1561 cctgatgcag ttgtcggagg gcgaagagag ccgagccttc agcttcgatg tcggcggacg
1621 cggctatgta ctgcgggtga atagctgcgc tgatggcttc tacaaagacc gctacgtgta
1681 ccgccacttc gccagcgctg cactacccat ccccgaagtg ttggacatcg gcgagttcag
1741 cgagagcctg acatactgca tcagtagacg cgcccaaggc gttactctcc aagacctccc
1801 cgaaacagag ctgcctgctg tgttacagcc tgtcgccgaa gctatggatg ctattgccgc
1861 cgccgacctc agtcaaacca gcggcttcgg cccattcggg ccccaaggca tcggccagta
1921 cacaacctgg cgggatttca tttgcgccat tgctgatccc catgtctacc actggcagac
1981 cgtgatggac gacaccgtgt ccgccagcgt agctcaagcc ctggacgaac tgatgctgtg
2041 ggccgaagac tgtcccgagg tgcgccacct cgtccatgcc gacttcggca gcaacaacgt
2101 cctgaccgac aacggccgca tcaccgccgt aatcgactgg tccgaagcta tgttcgggga
2161 cagtcagtac gaggtggcca acatcttctt ctggcggccc tggctggctt gcatggagca
2221 gcagactcgc tacttcgagc gccggcatcc cgagctggcc ggcagccctc gtctgcgagc
2281 ctacatgctg cgcatcggcc tggatcagct ctaccagagc ctcgtggacg gcaacttcga
2341 cgatgctgcc tgggctcaag gccgctgcga tgccatcgtc cgcagcgggg ccggcaccgt
2401 cggtcgcaca caaatcgctc gccggagcgc agccgtatgg accgacggct gcgtcgaggt
2461 gctggccgac agcggcaacc gccggcccag tacacgaccg cgcgctaagg aggtaggtcg
2521 agtttaaact ctagaaccgg tcatggccgc aataaaatat ctttattttc attacatctg
2581 tgtgttggtt ttttgtgtgt tcgaactaga tgctgtcgac cgatgccctt gagagccttc
2641 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
2701 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
2761 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
2821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
2881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
2941 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
3001 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
3061 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
3121 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
3181 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
3241 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
3301 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
3361 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
3421 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
3481 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
3541 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
3601 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
3661 atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag
3721 tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc
3781 tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca
3841 gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca
3901 gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg
3961 agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg
4021 gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct
4081 ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc
4141 tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta
4201 atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc
4261 ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc
4321 ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc
4381 gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg
4441 atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg
4501 gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa
4561 tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag
4621 tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa
4681 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact
4741 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
4801 cagtgcaagt gcaggtgcca gaacatttct ct
//
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